Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study

Figure 3

Phosphorylation of NFκB-p65 at Ser⁵³⁶ in lymphocytes after HBO exposure. (a) Level of t-NFκB-p65 protein in lymphocytes, normalized to β-actin and expressed as fold change before HBOT (arbitrary control set at 1); (b) level of p-NFκB-p65 (Ser⁵³⁶) protein in lymphocytes, normalized to β-actin and expressed as fold change before HBOT (arbitrary control set at 1); (c) ratio of p-NFκB-p65/t-NFκB-p65 expressed as fold change before HBOT (arbitrary control set at 1). Inset: representative Western blots (); ; ; p-NFκB-p65: the phosphorylated form of p65 subunit of nuclear factor-κB; t-NFκB-p65: the total form p65 subunit of nuclear factor-κB. Other abbreviations are under Table 1.

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