Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study

Figure 4

Phosphorylation of ERK1/2 at Thr²⁰² and Tyr²⁰⁴ in lymphocytes after HBO exposure. (a) Level of t-ERK1/2 protein in lymphocytes, normalized to β-actin and expressed as fold change before HBOT (arbitrary control set at 1); (b) level of p-ERK1/2/(Thr²⁰²/Tyr²⁰⁴) protein in lymphocytes, normalized to β-actin and expressed as fold change before HBOT (arbitrary control set at 1); (c) ratio of p-ERK1/2/t-ERK1/2 expressed as fold change before HBOT (arbitrary control set at 1). Inset: representative Western blots (); ; ; p-ERK1/2: the phosphorylated form of extracellular signal-regulated kinases 1 and 2; t-ERK1/2: the total form of extracellular signal-regulated kinases 1 and 2. Other abbreviations are under Table 1.

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