PDE5A Suppresses Proteasome Activity Leading to Insulin Resistance in C2C12 Myotubes

Figure 1

Effects of PDE5A on insulin signaling in C2C12 myotubes. (a) Representative western blots showing the insulin- (INS-) stimulated phosphorylation of Akt T308 and AS160 in the cells with PDE5A overexpression (OE); (b) quantification of phosphorylated Akt T308, phosphorylated AS160, and PDE5A protein in (a); (c) insulin-stimulated 2-DG uptake in the cells with PDE5A overexpression; (d) representative western blots showing the phosphorylation of Akt T308 and AS160 in the cells with PDE5A knockdown (KD); (e) quantification of phosphorylated Akt T308, phosphorylated AS160, and PDE5A protein in (d); (f) 2-DG uptake in the cells with PDE5A knockdown. . , vs. the indicated group.

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