PDE5A Suppresses Proteasome Activity Leading to Insulin Resistance in C2C12 Myotubes

Figure 2

Effects of PDE5A on ER stress in C2C12 myotubes. (a) Representative western blots showing the ER stress markers and the phosphorylation of JNK and IRS-1 S307 in the cells with PDE5A overexpression (OE); (b) quantification of the protein or phosphorylated protein levels in (a); (c) representative western blots showing the ER stress markers and the phosphorylation of JNK and IRS-1 S307 in the cells with PDE5A knockdown (KD); (d) quantification of protein or phosphorylated protein levels in (c). . , vs. the control group.

(a)

(b)

(c)

(d)