PDE5A Suppresses Proteasome Activity Leading to Insulin Resistance in C2C12 Myotubes

Figure 3

Effects of inhibition of ER stress on insulin signaling in PDE5A-overexpressed C2C12 myotubes. (a) Representative western blots showing the effects of 4-PBA on insulin- (INS-) stimulated phosphorylation of Akt T308 and AS160; (b) quantification of phosphorylated Akt T308 and AS160 in (a); (c) effects of 4-PBA on insulin-stimulated 2-DG uptake. (d) Representative western blots showing the effects of STF-083010 on insulin-stimulated phosphorylation of Akt T308 and AS160; (e) quantification of phosphorylated Akt T308 and AS160 in (d); (f) effects of STF-083010 on insulin-stimulated 2-DG uptake. . vs. the control group; vs. the insulin-treated group.

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