miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42/β-Catenin Signaling

Figure 1

Effects of miR-29a on MIN6 cells. (a) Effects of miR-29a mimic and inhibitor on miR-29a mRNA. RT-PCR to detect miR-29a mRNA expression after transient transfection with miR-29a mimic and inhibitor (n = 3). , compared with miR-29a NC group, as assessed by paired Student’s t-test. (b) Effects of miR-29a on MIN6 cell proliferation. CCK assay to detect proliferation after transfection with miR-29a mimic and inhibitor (n = 3). , compared with miR-29a NC group, as assessed by one-way ANOVA, followed by Fisher’s least significant difference test. (c and d) Effects of miR-29a on insulin secretion by MIN6 cells. ELISA to detect insulin secretion levels in MIN6 cells after transient transfection with miR-29a mimic and inhibitor under basal-glucose (5.0 mM) and high-glucose (20.0 mM) stimulation (n = 3). , compared with 5.0 mM glucose group, and , compared with miR-29a-NC group, as assessed by paired Student’s t-test. Data are shown as mean ± SD. NC: negative control.

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