miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42/β-Catenin Signaling

Figure 2

Effects of Cdc42 on MIN6 cells. (a) Effects of Cdc42-pcDNA3.1 on Cdc42 expression in MIN6 cells. Western blot to detect Cdc42 protein expression after transfection with Cdc42-pcDNA3.1 (n = 3). , compared with pcDNA3.1 group, as assessed by paired Student’s t-test. (b and c) Screening of Cdc42-siRNA fragments. (b) RT-PCR to detect Cdc42 mRNA expression after transient transfection with different siRNA fragments (n = 3). and , compared with siRNA-NC group, as assessed by paired Student’s t-test. (c) Western blot to detect Cdc42 protein expression after transient transfection with different siRNA fragments (n = 3). , compared with siRNA-NC group, as assessed by paired Student’s t-test. (d) Effects of Cdc42 on MIN6 cell proliferation. CCK assay to detect MIN6 cell proliferation after transfection with Cdc42-siRNA and Cdc42-pcDNA3.1 (n = 3). , compared with siRNA-NC group, , compared with pcDNA3.1 group, as assessed by one-way ANOVA, followed by Fisher’s least significant difference test. (e and f) Effects of Cdc42 on insulin secretion by MIN6 cells. ELISA to detect insulin secretion levels in MIN6 cells after transient transfection with Cdc42-pcDNA3.1 and Cdc42-siRNA under basal-glucose (5.0 mM) and high-glucose (20.0 mM) stimulation (n = 3). , compared with pcDNA3.1 group, , compared with siRNA-NC group, and , compared with 5.0 mM glucose group, as assessed by paired Student’s t-test. Data are shown as mean ± SD. NC: negative control.

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