Comparative Proteome Analysis of Epicardial and Subcutaneous Adipose Tissues from Patients with or without Coronary Artery Disease

Figure 1

Workflow of differential proteomic analysis of EAT and SAT samples from CAD and non-CAD patient populations. Epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT) samples were collected from 6 CAD patients and 6 non-CAD patients. Total proteins of adipose tissue were extracted and quantified via iTRAQ-coupled 2D LC-MS/MS. The differentially expressed proteins between the CAD and non-CAD groups were identified and analyzed by MASCOT and IPA (Ingenuity Pathway Analysis Software). Western blot was used to verify the selected differentially expressed proteins.