Wingless-Type MMTV Integration Site Family Member 5a Is a Key Secreted Islet Stellate Cell-Derived Product that Regulates Islet Function

Figure 1

The effects of different ISC profiles on islet insulin secretory function and Wnt5a content and release by ISCs. (a) Compared with coculture with ISCs isolated from normoglycemic db/m mice, coculture of islets with ISC isolation from db/db mice significantly reduced insulin secretion in vitro. All data were expressed as (), and , normoglycemic db/m ISCs vs. hyperglycaemic db/db ISCs. (b) Protein levels of Wnt5a were increased in control db/m ISCs than db/db ISCs. All data were expressed as (), and , normoglycemic db/m ISCs compared with hyperglycaemic db/db ISCs. (c) Wax-embedded sections of db/m and db/db mouse pancreases showing the expression of Wnt5a and Frizzled 5 as revealed by immunohistochemistry. Scale . (d) Protein levels of Wnt5a were increased in db/m ISCs lysates and supernatant than in the db/db ISCs. All data were expressed as (), and , normoglycemic db/m ISCs compared with hyperglycaemic db/db ISCs.

(a)

(b)

(c)

(d)