Wingless-Type MMTV Integration Site Family Member 5a Is a Key Secreted Islet Stellate Cell-Derived Product that Regulates Islet Function

Figure 2

Control db/m ISCs and Wnt5a increase insulin secretion with no change in cytokine-induced apoptosis and viability. (a) The in vitro insulin was significantly increased by coculture with 0.05 μg/ml rhWnt5a protein for 48 hours compared to coculture with the control (static). All data were expressed as (), and , db/m islets vs. db/m islets +0.05 μg/ml Wnt5a. (b) The in vitro insulin secretion was significantly increased by coculture with 0.05 μg/ml rhWnt5a protein for 48 hours compared to coculture with the control (perifusion). All data were expressed as (), and , db/m islets vs. db/m islets +0.05 μg/ml Wnt5a. (c) Assay of apoptosis in islets from the cytokine-induced and control groups. There was no change in the level of apoptosis. (d) Assay of viability in islets from the cytokine-induced and control groups. There was no change in the level of viability.

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