Identification of Serum miRNA-423-5p Expression Signature in Somatotroph Adenomas

Figure 5

Effect of miR-423-5pon cell growth, apoptosis, growth hormone release, and migration of GH3 cells. (a): Viability of GH3 cells after miR-423-5ptransfection for 24h, 48h, and 72h. (b): miR-423-5p induced GH3 cell apoptosis after transfection for 24 h. (c): miR-423-5p reduced growth hormone release by GH3 cell cultures. (d): Transwell experiments showing miR-423-5p reduced transmembrane GH3 cells after transfection for 24h. (e): miR-423-5p inhibited mRNA of migration-related genes N-CAD, vimentin, VEGF, and MMP2 compared to a control group. < 0.05, < 0.01, #compared to miR-423-5p p<0.05, n = 3.

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