A Novel Pentapeptide Inhibitor Reduces Amyloid Deposit Formation by Direct Interaction with hIAPP

Figure 2

Quantitative analysis of the inhibitory effects of FLPNF and NFGAIL on hIAPP aggregation formation by Thioflavin T (ThT) staining. (a) ThT fluorescence of each group was observed at 0, 12, 24, and 48 hours using a fluorescence microscope (“control” represented PBS solution, “hIAPP” represented 10 μM hIAPP in PBS, “hIAPP+FLPNF” represented 10 μM hIAPP with 100 μM FLPNF in PBS, and “hIAPP+NFGAIL” represented 10 μM hIAPP with 100 μM NFGAIL in PBS; 20 μM ThT was added to each group; scale bar: 50 μm). (b) ThT fluorescence intensity values were measured by a luminescence meter at 0, 12, 24, and 48 hours (values were presented as ; each experiment was repeated thrice; significantly different compared to hIAPP, unpaired -test, ; significantly different compared to hIAPP, unpaired -test, ).

(a)

(b)