A Novel Pentapeptide Inhibitor Reduces Amyloid Deposit Formation by Direct Interaction with hIAPP

Figure 3

Analysis of inhibitory effects of different concentrations of FLPNF and NFGAIL on hIAPP aggregation formation by ThT staining. 10 μM hIAPP incubated with 0, 20, 50, 100, 200, and 400 μM FLPNF or NFGAIL in PBS for 24 hours at 37°C. (a) ThT fluorescence of each group was observed using a fluorescence microscope (stained by 20 μM ThT; scale bar: 50 μm). (b) The fluorescence intensity values of each group were measured by a luminescence meter for 24 hours (values were presented as ; each experiment was repeated thrice; significantly different compared to 0 μM, unpaired -test, ).

(a)

(b)