Research Article

GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells

Figure 1

ADMSC transfection and differentiation. (a) GFP expression in OEG, V1, InG, and V2, visualized by fluorescence microscopy. (b) Quantitative RT-PCR analysis of the expression levels of GNPDA2. The relative expression is shown as the means ± standard deviation of three independent experiments made in triplicates. . (c) Western blot analysis of the protein levels of GNPDA2. (d) Oil Red O staining after 10 days of culture in adipocyte differentiation inductive medium. OEG: GNPDA2 overexpressed ADMSCs; V1: the control cells of OEG; InG: GNPDA2 shRNA knockdown ADMSCs; V2: the control cells of InG.
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