Hyperoside Alleviates High Glucose-Induced Proliferation of Mesangial Cells through the Inhibition of the ERK/CREB/miRNA-34a Signaling Pathway

Figure 2

Hyperoside suppresses miR-34a expression via the inhibition of ERK activation. (a) SV40-MES13 cells were treated with LG or HG with different concentration of hyperoside for 48 h. The phosphorylation level of ERK was examined by Western blot. (b) SV40-MES13 cells treated with or without U0126 (10 μM) were concurrently treated with or without hyperoside (200 μM) for 48 h under HG condition. The phosphorylation level of ERK was determined by Western blotting. ((c), (d)) Cell proliferation was determined by CCK-8 and the EdU incorporation assay. (e) The expression of miR-34a was detected by qRT-PCR (, , , n.s. indicates no significance).

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