Hyperoside Alleviates High Glucose-Induced Proliferation of Mesangial Cells through the Inhibition of the ERK/CREB/miRNA-34a Signaling Pathway

Figure 4

CREB-mediated transcriptional regulation of miR-34a. (a) Binding region sequence between CREB and miR-34a promoter was obtained by the ChIP assay and DNA sequencing. (b) Binding sites of CREB in the miR-34a promoter region were predicted according to an online prediction. (c) Schematic diagram of the putative CREB-binding sites in the promoter region of miR-34a. (d) Relative activities of luciferase reporters carrying the binding sites of CREB in the miR-34a promoter region that were cotransfected with either pcDNA3.1-HA-CREB or pcDNA3.1 empty vector into 293T cells.

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