GLP-1/GLP-1R Signaling Regulates Ovarian PCOS-Associated Granulosa Cells Proliferation and Antiapoptosis by Modification of Forkhead Box Protein O1 Phosphorylation Sites

Figure 3

Proliferation and antiapoptosis of granulosa cells of PCOS mice were promoted by GLP-1 (7-36) in vitro. (a) GCs were isolated from the ovaries of PCOS model mice (day 54 of life) and treated with increasing doses of GLP-1 (7-36) (0, 10, 100, and 1000 nM). Cell viability was measured by CCK-8 assay. (b) Flow cytometry Annexin V-FITC/PI double staining was used to detect the apoptotic rate of PCOS MGCs in control group and GLP-1 (7-36) (10 nM, 100 nM, and 1000 nM) treated for 48 hours. (c) Bcl-2, bax, and beta-actin were detected using their specific antibodies by the Western blot analysis for the PCOS MGCs with or without 100 nM GLP-1 (7-36) interference for 48 hours. Results are expressed in at least three separate experiments. , and .

(a)

(b)

(c)