GLP-1/GLP-1R Signaling Regulates Ovarian PCOS-Associated Granulosa Cells Proliferation and Antiapoptosis by Modification of Forkhead Box Protein O1 Phosphorylation Sites

Figure 4

GLP-1 (7-36) induced phosphorylation of FoxO1 in PCOS ovarian MGCs. The isolated PCOS mice ovarian GCs were pretreated in the presence or absence of 100 nM GLP-1 (7-36) to detect FoxO1, pFoxO1 (Ser 256), and pFoxO1 (ser 391) using their specific antibodies by the Western blot analysis. The experiment was repeated thrice.