GLP-1/GLP-1R Signaling Regulates Ovarian PCOS-Associated Granulosa Cells Proliferation and Antiapoptosis by Modification of Forkhead Box Protein O1 Phosphorylation Sites

Figure 5

GLP-1 (7-36) protected PCOS MGCs by modification of forkhead box protein O1 phosphorylation sites. (a) FoxO1 was detected using their specific antibodies by the Western blot analysis for the PCOS MGCs after transfection. (b) FoxO1, pFoxO1 (ser 256), bcl-2, and bax were detected using their specific antibodies by the Western blot analysis for the PCOS MGCs treated with 100 nM GLP-1 (7-36) for 48 h after transfection as indicated in the figure legends. The experiment was repeated thrice.

(a)

(b)