Research Article

Fetal-Perinatal Exposure to Bisphenol-A Affects Quality of Spermatozoa in Adulthood Mouse

Figure 3

Flow cytometry analysis of caput and cauda spermatozoa (SPZ) from mice exposed to vehicle (control, CTRL) or Bisphenol-A (BPA). (a) Representative histograms of Acridine orange (AO) stained sperm in M1- and M2-gated areas. Intensely green (FL1-H > 105), green (FL1-H > 103), red (FL3-H > 103), and total (green + red) fluorescencing DNA were used to analyze (b) high DNA stainability (HDS) and (c) thiol/disulphide status (TDS) values. Graphs were representative of three sperm samples relative to separate animals. Data were expressed as the mean ± S.E.M. and .
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