Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats

Figure 8

Human normal thyroid epithelial cells were isolated and cultured. The cells were treated with and without palmitic acid (PA) in the presence and absence of a desuccinylation inhibitor (NAM) for 24 hours, respectively. Total proteins of each group were subjected to immunoprecipitation (IP) with an antibody of isocitrate dehydrogenase 2 (IDH2). Succinylation status and expression of IDH2 were evaluated by Western blotting analysis. IDH2 expression showed no obvious change. IDH2 succinylation was inhibited by PA treatment and showed no obvious change in response to NAM alone. After treatment of both PA and NAM, IDH2 succinylation level was recovered compared with PA treatment.