The effect i.p. injection of 10 nmol/mouse spexin on Npy, Agrp, Pomc, Cart, Crh, Orexin, and Mch mRNAs levels in the hypothalamus (a) and brainstem (b) in mice. The housekeeping gene 36B4 was used as the internal control. The target genes were detected by RT-qPCR after 10 nmol spexin injection. The unpaired t-test was utilized to analyze the difference between the spexin and the control (NS). Data are presented as mean ± S.E.M. n = 6−8 per group. versus the control.
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