Dysregulation in the Unfolded Protein Response in the FGR Rat Pancreas

Figure 2

Expression of unfolded protein response- (UPR-) related factors in the FGR fetal pancreas, as determined by qPCR arrays. (a) K-means clustering representation of eighty-four mRNA profiles. The magnitude of the change is represented by a color scale (top right) from low (green) to high (red). b and c indicates scatter plot and volcano plot displaying differentially expressed genes in pancreatic tissues between two groups (n = 3). (b) The x and y axis, respectively, indicate the mean expression value of control and FGR after log₁₀ transformation. (c) The y-axis indicates the value after log₁₀ transformation, and the x-axis displays the log2 fold change (FC) value (FGR/control). Red dots represent upregulated transcripts (FC > 2); blue dots represent downregulated transcripts; black dots represent unchanged transcripts (0.5 < FC < 2). (d) Differentially expressed genes divided into 5 categories according to molecular function. TF, transcription factor; ERAD, endoplasmic reticulum-associated degradation; AP, apoptosis, HSPs, heat-shock protein; RT, regulation of translation. (e) Differentially expressed genes between two groups (n = 3 per group; vs. control, unpaired Student’s t-test).