Haptoglobin Phenotypes Are Associated with the Postload Glucose and Insulin Levels in Pediatric Obesity

Figure 1

Hp-phenotypization by Western immunoblot in plasma samples of obese pediatric subjects. Diluted plasma samples from six representative obese pediatric subjects were analyzed by WIB under reduced condition to identify the Hp phenotype using a specific antibody against the α-chain. The band ∼9 kDa corresponds to the α¹-chain, whereas the band at ∼20 kDa corresponds to the α²-chain. In the presence of only one band, 20 kDa or 9 kDa, the phenotype was, respectively, 2-2 and 1-1; when both bands were detectable, the phenotype was heterozygous 1-2.