Liraglutide Promotes Osteoblastic Differentiation in MC3T3-E1 Cells by ERK5 Pathway

Figure 10

XMD8-92 downregulated the Col-1, OPG, and OCN gene expression. The gene expression of Col-1, OPG, and OCN was detected by RT-PCR after the MC3T3-E1 cells were pretreated with 10 μM XMD8-92 for 1 h and followed by 100 nM liraglutide treatment for 48 h. Quantitative analysis of the gene level of Col-1 (a), OPG (b), and OCN (c) in MC3T3-E1 cells after being pretreated with 10 μM XMD8-92 for 1 h followed by 100 nM liraglutide treatment for 48 h.  < 0.01 compared with control,  < 0.01 compared with 100 nM liraglutide, and  < 0.01 compared with XMD8-92 group. C: control group; LIR: 100 nM liraglutide; X: XMD8-92; X + LIR: XMD8-92 with 100 nM liraglutide.

(a)

(b)

(c)