Liraglutide Promotes Osteoblastic Differentiation in MC3T3-E1 Cells by ERK5 Pathway

Figure 8

XMD8-92 inhibited the activity of the ERK5 signaling pathway. The protein expression of MEK5 and NUR77 was detected by western blot after the MC3T3-E1 cells were pretreated with 10 μM XMD8-92 for 1 h followed by 100 nM liraglutide treatment for 48 h (a). Quantitative analysis of the protein level of MEK5 (b) and NUR77 (c) in MC3T3-E1 cells after being pretreated with 10 μM XMD8-92 for 1 h followed by 100 nM liraglutide treatment for 48 h,  < 0.05 compared with control,  < 0.05 compared with 100 nM liraglutide, and  < 0.05 compared with XMD8-92 group. C: control group; LIR: 100 nM liraglutide; X: XMD8-92, X + LIR: XMD8-92 with 100 nM liraglutide.

(a)

(b)

(c)