A Novel Homozygous Mutation of Thyroid Peroxidase Gene Abolishes a Disulfide Bond Leading to Congenital Hypothyroidism

Figure 2

Comparison of amino acid sequences with various mammalian species amino acid sequences containing the novel mutations was compared with those in other mammalian species. ClustalW calculates the best match for selected sequences and lines them up so that identities and differences can be observed. The arrow indicates the position of the amino acid substitution Cys655Phe. The asterisks show perfectly preserved amino acids. The colons show high homology. The dots show low homology. The amino acid sequences are based on Ensembl, which is a genome browser for vertebrate genomes, and NCBI, which houses a series of databases relevant to biotechnology and biomedicine and is an important resource for bioinformatics tools and services. Human TPO (ENST00000329066.8), mouse TPO (ENSMUST00000021005.14), rat TPO (ENSRNOT00000006526.3), rabbit TPO (ENSOCUT00000016422.3), xenopus (tropical clawed flag) TPO (ENSXETT00000002202.1), and zebrafish (Danio rerio) TPO (XP_021322945.1).