Resistin Activates p65 Pathway and Reduces Glycogen Content through Keratin 8

Figure 4

Resistin regulates K8 transcription through p65. (a) HepG2 cells were treated with or without resistin and K8 expression detected by using qRT-PCR. (b) HepG2 cells were transfected with a plasmid overexpressing p65 and K8 expression assessed by using qRT-PCR. (c) HepG2 cells were transfected with siRNA-p65 and p65 expression was determined by using qRT-PCR. (d) HepG2 cells were treated with or without resistin for 24 h after transfection with siRNA-p65, and K8 mRNA levels were determined by using qRT-PCR. (e) HepG2 cells were transfected with p65, and the glycogen content was determined after 24 h. (f) HepG2 cells were cotransfected with K8 siRNA and the p65 expression plasmid, and the cellular glycogen content was measured after 24 h. Data are presented as mean ± SD. and .

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