Recurrent Germline Mutations of CHEK2 as a New Susceptibility Gene in Patients with Pheochromocytomas and Paragangliomas

Table 2

PCR primers for CHEK2 mutations in somatic DNA from FFPE tissues.

Primer	Upstream	Downstream
2S300	CACTGAGCTCCTTAGAGAC	CAAGATTGGCAAATCCATC
6S770	TTTGTTTTTCCCTCTAGTGGT	ATTATTTTGGGAAGTTATGAAG
9S41980	GAGCTGTTTGACAAAGTGGT	GTTTTAATCCACGGTCCCT

(a) The condition of PCR amplification was as follows: predenaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 56°C/52°C/56°C for 30 s, and extension at 72°C for 30 s. A total of 35 cycles were carried out, final extension at 72°C for 10 min. (b) PCR products were identified by 1.5% agarose gel electrophoresis and sent to the Beijing SinoGenoMax Company for purification and sequencing. The sequencing was performed by ABI 3730XL instrument.