Effect of Supplementation of Fermented Yeast Culture on Hormones and Their Receptors on Exposure to Higher Temperature and on Production Performance after Exposure in Nicobari Chickens

Table 2

Sequence of the primers.

Genes	Sequence of forward (F) and reverse (R) primers	Size of the PCR product (bp)	Annealing temperature (oC)
Leptin R	F 5′-CGGACTACCTCATGAAGATCCTGAC-3′	162	55
	R 5′-GCCAATGGTGATGACCTGACCATC-3′
GSHR	F 5′-CTGCAAGCTCTTCCAGTTCATCAGC-3′	158	55
	R 5′-CCAGAGGATGAGGATGACCAGCTTG-3′
GH R	F 5′-TCAGAAAGGATGGATTACTCTGGAGTATG -3′	161	55
	R 5′-CGGAGGTACGTTGTCTTGATCGGAC-3′
Actin β	F 5′-CGGACTACCTCATGAAGATCCTGAC-3′	197	55
	R 5′-GCCAATGGTGATGACCTGACCATC-3′