G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner

Figure 1

Inflammatory response before and after MR knockdown with different aldosterone concentrations. (a) MR mRNA expression; (b) level of cell culture medium IL-1β; (c) ICAM-1 and VCAM-1 mRNA expressions; (d) protein level of IL-1β; (e) protein level of NLRP-3; (f) Western blot of IL-1β and NLRP-3. scRNA: the state with control RNA, siMR: the state after MR knockdown, aldo: aldosterone. value < 0.05 compared between scRNA and siMR with the same aldosterone concentration, value < 0.01 compared between scRNA and siMR with the same aldosterone concentration, value < 0.001 compared between scRNA and siMR with the same aldosterone concentration. ns: nonsignificant.

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