TNRC6C Functions as a Tumor Suppressor and Is Frequently Downregulated in Papillary Thyroid Cancer

Figure 3

TNRC6C regulates the proliferation, apoptosis, migration, and invasion abilities of BCPAP cells. (a) BCPAP cells were transfected with TNRC6C-siRNA1, TNRC6C-siRNA2, TNRC6C-siRNA3, and control-SiRNA, respectively. The relative mRNA expression of TNRC6C was quantified by real-time qPCR. (b) BCPAP cells were transfected with pcDNA3.1-TNRC6C plasmid and pcDNA3.1-empty plasmid, respectively. The relative mRNA expression of TNRC6C was quantified by real-time qPCR. (c) Growth curve of BCPAP cells determined by CCK8 assay after transfection with TNRC6C-SiRNA3 or control-SiRNA. (d) Growth curve of BCPAP cells determined by CCK8 assays after transfection with pcDNA3.1-TNRC6C plasmid or pcDNA3.1-empty plasmid. (e) The effect of TNRC6C knockdown or overexpression on the migration of BCPAP cells was assessed using wound-healing assay. Quantitative analysis (left) and representative images (right). (f) The effect of TNRC6C knockdown or overexpression on BCPAP cell apoptosis was determined by flow cytometry assay. (g) The effect of TNRC6C knockdown or overexpression on the migration of BCPAP cells was assessed using transwell migration assay. Quantitative analysis (left) and representative images (right). (h) The effect of TNRC6C knockdown or overexpression on the invasion of BCPAP cells was assessed using transwell invasion assays (quantitative analysis (left) and representative images (right). Values represent the mean ± SD from three independent experiments; empty-plasmid, pcDNA3.1-empty plasmid; TNRC6C-plasmid, pcDNA3.1-TNRC6C plasmid; ; ; ; .

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