Resveratrol Alleviates Skeletal Muscle Insulin Resistance by Downregulating Long Noncoding RNA

Figure 6

Resveratrol improved skeletal muscle insulin resistance by downregulating the lncRNA NONMUT044897.2 in vitro. Validation of lncRNAs NONMMUT044897.2 (a), NONMMUT139818.1 (b), NONMMUT00000181045(c), NONMMUT071570.2 (d), and NONMMUT06516.2 (e) by RT-qPCR. Expression of miR-7051-5p mRNA (f) and NONMMUT044897.2 mRNA (g) after shRNA transfection into C2C12 cells in different groups. (h) Glucose concentrations in the culture medium after shRNA transfection into C2C12 cells in different groups. (i) Protein bands of insulin signaling pathway-related molecules. Densitometric analysis of (j) AKT, (k) p-AKT, (l) GSK3β, (m) p-GSK3β, (n) GLUT4, and (o) SOCS1. Data are presented as the mean ± SD (n = 6). versus CON, versus PA, and versus PA + shRNA-NONMMUT044897.2.

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