TET3 Mediates 5hmC Level and Promotes Tumorigenesis by Activating AMPK Pathway in Papillary Thyroid Cancer

Figure 2

(a) Western blot verified the expression level of TET3 was decreased with shTET3 intervention in BCPAP and TPC-1 cells; (b) the results of immunofluorescence showed that inhibiting the expression of TET3 significantly decreased the 5hmC level of papillary thyroid carcinoma cell lines BCPAP and TPC-1. Upper panel: 5hmC staining (green); lower panel: DAPI staining (blue), superimposed with 5hmC staining; (c) CCK-8 assays and (d) colony formation assays showed that TET3 knockdown significantly suppressed cell proliferation in BCPAP and TPC-1 cells.

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