Blocking REDD1/TXNIP Complex Ameliorates HG-Induced Renal Tubular Epithelial Cell Apoptosis and EMT through Repressing Oxidative Stress

Figure 2

Expression of REDD1 in the kidneys of diabetic mice and HK-2 cells. (a) Immunohistochemical staining analysis of the expression of REDD1 in db/db mice and STZ-induced diabetic mice (scale bar, 50 μm). (b, c) Western blot of REDD1 in the renal tissues. (d, e) Western blot of REDD1 in HK-2 cells were treated with high glucose (30 mM) at varying time points. (f, g) Immunofluorescence analysis of REDD1 expression in HK-2 cells. Values are expressed as means ± SD (n = 5 per group). NG: normal glucose group, 5.6 mM D-glucose; M: hypertonic control group, NG + 24.4 mM mannitol; HG: high glucose group, 30 mM D-glucose. versus NG.

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