Research Article
Improvement of Skin Wound Healing for Diabetic Mice with Thermosensitive Hydrogel Combined with Insulin Injection
Table 1
Total RNA reverse transcription system.
| | Component | Volume (μL) | Final concentration |
| | dNTP mix, 2.5 mM | 4 | 500 μM | | Primer mix | 2 | — | | RNA template | X | 50 pg–5 μg | | 5×RT buffer | 4 | 1× | | DTT, 0.1 M | 2 | 10 mM | | HiFiScript, 200 U/μL | 1 | — | | RNase-free water | Up to 20 | — |
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