Testosterone Deficiency Promotes Hypercholesteremia and Attenuates Cholesterol Liver Uptake via AR/PCSK9/LDLR Pathways

Table 1

Primer sequences for real-time quantitative RT-PCR.

Gene name	Species	Sequence		Size (bp)	GenBank accession no.
β-actin	Human	Forward	5′-GCCGCCAGCTCACCAT-3′	171	NM001101
		Reverse	5′-AGGAATCCTTCTGACCCATGC-3′
β-actin	Rat	Forward	5′-GATATCGCTGCGCTCGTCG-3′	217	NM031144
		Reverse	5′-CAATGCCGTGTTCAATGGGG-3′
LDLR	Human	Forward	5′-ATTGTCCTCCCCATCGTGCT-3′	164	NM000527
		Reverse	5′-CTGTAGCCGTCCTGGTTGTG-3′
Ldlr	Rat	Forward	5′-TTGGCTGCATCAATGTGACC-3′	155	NM175762
		Reverse	5′-CAAGCACTCGTTGGTCTTGC-3′
PCSK9	Human	Forward	5′-GGTTAGCGGCACCCTCATAG-3′	231	NM174936
		Reverse	5′-CCAACTGTGATGACCTCGGG-3′
Pcsk9	Rat	Forward	5′-AAGATAGCTCCCCTGACGGA-3′	170	NM199253
		Reverse	5′-ATGGCTGTCACACTTGCTCG-3′

LDLR/Ldlr, low-density lipoprotein receptor; PCSK9/Pcsk9, proprotein convertase subtilisin/kexin type 9.