Exosomal Circular RNA hsa_circ_0046060 of Umbilical Cord Mesenchymal Stromal Cell Ameliorates Glucose Metabolism and Insulin Resistance in Gestational Diabetes Mellitus via the miR-338-3p/G6PC2 Axis

Figure 1

The identification of hUMSCs and hUMSC-EXOs and characterization of hsa_circ_0046060. (a) Flow cytometry analysis showing high expression levels of CD90 and CD105 and negative expression of CD45 and CD34 in hUMSCs. (b) Representative TEM micrographs of hUMSC-Exos characterization. Scale bar: 100 nm. (c) NTA indicating the particle size and concentration of exosomes. (d) Western blot analysis indicating expression of specific markers of huMSC-EXOs. (e) Agarose gel electrophoresis photograph of RT-qPCR assay showing amplification of hsa_circ_0046060. (f) Sanger sequencing analysis using specific primers for hsa_circ_0046060 indicating the splicing site. (g) FISH assay showing localization of hsa_circ_0046060 in the cytoplasm of hUMSCs. (h) RT-qPCR analysis of has_ circ_0046060 and its host gene mRNA treated with or without RNase R Scale bar: 50 μm. (i) has_circ_0046060 expression levels in C-hUCB-EXOs, C-hUMSC, C-hUMSC-EXOs, G-hUCB-EXOs, G-hUMSC and G-hUMSC-EXOs as determined by qRT-PCR (, , , vs. C-hUCB-EXOs; ^ , ^  ^ , ^  ^  ^  ^ , vs. C-hUMSC; ^##, ^####, vs. C-hUMSC-EXOs; ^&&&, ^&&&&, vs. G-hUCB-EXOs; ^@@, G-hUMSC vs. G-hUMSC-EXOs).

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