Exosomal Circular RNA hsa_circ_0046060 of Umbilical Cord Mesenchymal Stromal Cell Ameliorates Glucose Metabolism and Insulin Resistance in Gestational Diabetes Mellitus via the miR-338-3p/G6PC2 Axis

Figure 2

Hsa_circ_0046060 inhibition reversed the effect of GDM-derived hUMSC exosome on intracellular glucose concentration. (a) Inhibitory effects of three candidate siRNAs targeting hsa_circ_0046060 as determined by RT-qPCR , , , vs. siNC; ^{^}, vs. si-circRNA1; ^##, si-circRNA2 vs. si-circRNA3). (b) Effect of lentiviral system on hsa_circ_0046060 expression level in hUMSCs and hUMSC-EXOs as determined by RT-qPCR. , . (c) Fluorescence microscopy showing intake of PKH67 labeled exosomes into L-02 cells after 24 h treatment. Scale bar = 20 μm. (d) Levels of hsa_circ_0046060 were determined using RT-qPCR after treatment with different huMSC-EXOs (, , , vs. control; ^  ^ , vs. C-hUMSC-EXOs; ^###, vs. G-hUMSC-EXOs; ^&&&, siNC-G-hUMSC-EXOs vs. sh-circ-G-hUMSC-EXOs). (e) Glucose level in L-02 cells as determined using the glucose content assay kit (, , vs. control; ^{^}, vs. C-hUMSC-EXOs; ^##, vs. G-hUMSC-EXOs; ^&, siNC-G-hUMSC-EXOs vs. sh-circ-G-hUMSC-EXOs).

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