Exosomal Circular RNA hsa_circ_0046060 of Umbilical Cord Mesenchymal Stromal Cell Ameliorates Glucose Metabolism and Insulin Resistance in Gestational Diabetes Mellitus via the miR-338-3p/G6PC2 Axis

Figure 3

Characterization of hsa_circ_0046060 in hUMSCs and hUMSC-EXOs. (a) Blast analysis to explore homology between human circRNA sequence XXX and mice sequence. (b, d) Bodyweight of pregnant and fetal mice in control and GDM groups as determined by an electronic scale. (c, e) Fasting blood glucose level of pregnant and fetal mice in control and GDM groups as determined using blood glucose test strips. (f) Expression levels of exosomal mmu_circ_0002819 in control and GDM mice as determined using RT-qPCR. (g) Inhibitory effects of three siRNA-mediated knockdown of mmu_circ_0002819 as determined by RT-qPCR assay. (h) Effect of lentiviral sh-mmu_circ_0002819 on mmu_circ_0002819 expression in hUMSCs and hUMSC-EXOs as evaluated by RT-qPCR. (i) Sanger sequence analysis showing the splicing site. (j) RT-qPCR products were evaluated using agarose gel electrophoresis. , .

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