Exosomal Circular RNA hsa_circ_0046060 of Umbilical Cord Mesenchymal Stromal Cell Ameliorates Glucose Metabolism and Insulin Resistance in Gestational Diabetes Mellitus via the miR-338-3p/G6PC2 Axis

Figure 4

Si-circ-G-mUMSC-EXOs ameliorated glucose and insulin resistance in GDM mice. (a) Inhibitory effects of three potential siRNAs targeting mmu_circ_0002819 as determined by RT-qPCR assay (, si-mmu_circRNA1, mmu_si-circRNA2 and mmu_si-circRNA3 vs. Control; ^###, si-mmu_circRNA1, mmu_si-circRNA2 and mmu_si-circRNA3 vs. siNC). (b) Inhibitory effect of sh-mmu_circ_0002819 on mmu_circ_0002819 expression in mUMSCs and mUMSC-EXOs as evaluated by RT-qPCR. , (c) Serum glucose level in mice from the five groups following glucose tolerance test (, , vs. Control; ^ , ^  ^, GDM vs. GDM + si-circ-G-mUMSC-EXOs; ^##, GDM + G-mUMSC-EXOs vs. GDM + si-circ-G-mUMSC-EXOs). (d) Serum glucose level of mice in the five groups following insulin tolerance test (, , vs. Control; ^{^}, ^{^^}, GDM vs. GDM + si-circ-G-mUMSC-EXOs; ^##, ^###, GDM + G-mUMSC-EXOs vs. GDM + si-circ-G-mUMSC-EXOs). (e) Expression level of mmu_circ_0002819 in mice liver as evaluated using RT-qPCR (, , vs. Control; ^  ^  ^, GDM vs. GDM + si-circ-G-mUMSC-EXOs; ^###, GDM + G-mUMSC-EXOs vs. GDM + si-circ-G-mUMSC-EXOs). (f) Glucose concentration as determined using Glucose Assay kit (, , , vs. Control; ^{^}, PA + C-hUMSC-EXOs vs. PA + G-hUMSC-EXOs; ^##, PA + insulin + C-hUMSC-EXOs vs. PA + insulin + G-hUMSC-EXOs; ^&&, PA + insulin + G-hUMSC-EXOs vs. PA + insulin + si-circ-G-hUMSC-EXOs). (g) Glycogen levels as determined using Glycogen Content Assay Kit (, , vs. Control; ^{^}, PA + C-hUMSC-EXOs vs. PA + G-hUMSC-EXOs; ^#, PA + insulin + C-hUMSC-EXOs vs. PA + insulin + G-hUMSC-EXOs; ^&, PA + insulin + G-hUMSC-EXOs vs. PA + insulin + si-circ-G-hUMSC-EXOs). (h) Expression levels of p-IRS-1 and IRS-1 in the L-02 cells as determined by western blot. (i) Quantification of p-IRS-1 and IRS-1 in different groups (, , vs. Control; ^#, PA + insulin + C-hUMSC-EXOs vs. PA + insulin + G-hUMSC-EXOs; &&, PA + insulin + G-hUMSC-EXOs vs. PA + insulin + si-circ-G-hUMSC-EXOs).

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