Exosomal Circular RNA hsa_circ_0046060 of Umbilical Cord Mesenchymal Stromal Cell Ameliorates Glucose Metabolism and Insulin Resistance in Gestational Diabetes Mellitus via the miR-338-3p/G6PC2 Axis

Figure 5

Hsa-miR-338-3p was directly bound to hsa_circ_0046060 and it regulated G6PC2 expression. (a) hsa-miR-338-3p targets the 3′-UTR sequence of hsa_circ_0046060. Luciferase reporter assay was performed to verify the interaction between hsa_circ_0046060 and hsa-miR-338-3p in L-02 cells. . Effects of hsa-miR-338-3p inhibitor on the expression of (b) hsa-miR-338-3p (, vs. Control; ^  ^  ^  ^ , vs. siNC-G-hUMSC-EXOs; ^####, si-circ-G-hUMSC-EXOs vs. si-circ-G-hUMSC-EXOs + inhibitor miR; ^&&&&, si-circ-G-hUMSC-EXOs vs. si-circ-G-hUMSC-EXOs + inhibitor miR) and (c) G6PC2 (, , , vs. Control; ^  ^  ^  ^, vs. siNC-G-hUMSC-EXOs; ^##, si-circ + G-hUMSC-EXOs vs. si-circ-G-hUMSC-EXOs + inhibitor miR; ^&&, si-circ-G-hUMSC-EXOs vs. si-circ-G-hUMSC-EXOs + inhibitor miR) as determined by RT-qPCR assay. (d) hsa-miR-338-3p targets the 3′-UTR sequence of G6PC2. The relative luciferase activity was evaluate to verify the interaction between G6PC2 and hsa-miR-338-3p in L-02 cells. .

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