LncRNA X Inactive Specific Transcript Exerts a Protective Effect on High Glucose-Induced Podocytes by Promoting the Podocyte Autophagy via miR-30d-5p/BECN-1 Axis

Figure 1

Detection of biochemical indexes, pathological changes, and autophagy-related proteins in DN mice and normal mice. C57BL/6 mice were treated with 50 mg/kg STZ in citrate buffer (DN group) for 5 consecutive days or treated with citrate buffer (control group). (a) The whole-blood GLU was measured using a blood GLU meter. (b) The urinary protein concentration was determined by the corresponding kit. (c) and (d) SCR and BUN levels were detected using an automatic biochemical analyzer. (e) Hematoxylin and eosin staining of kidney tissues (magnification: 100x times; scale bar = 100 μm). (f) PAS staining of kidney tissues (magnification: 100x times; scale bar = 100 μm). (g) Renal podocytes were observed by a transmission electron microscope (magnification: 7000x times; scale bar = 1 μm). (h) Immunohistochemistry was used to detect the expression of LC3 in kidney tissues (magnification 100x times; scale bar = 100 μm). (i)–(l) The protein levels of Beclin 1, LC3 I/II, and in kidney tissues were detected by Western blot. GAPDH was used as the internal control. Quantified values were presented as mean ± standard deviation of at least three independent experiments. , vs. control. STZ: streptozotocin. DN: diabetic nephropathy. GLU: glucose. SCR: serum creatinine. BUN: blood urea nitrogen. GAPDH: glyceraldehyde-3-phosphate dehydrogenase. PAS: periodic acid Schiff.

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