LncRNA X Inactive Specific Transcript Exerts a Protective Effect on High Glucose-Induced Podocytes by Promoting the Podocyte Autophagy via miR-30d-5p/BECN-1 Axis

Figure 2

Validation of the targeting relationship of XIST/BECN1 and miR-30d-5p. (a) The miRDB (https://mirdb.org/index.html) and TargetScan (https://www.targetscan.org/vert_72/) were used to search the miRNAs targeting BECN1. The results were intersected by a Venn diagram. (b) The binding sites between miR-30d-5p and BECN1 were predicted by TargetScan. (c) The relationship between miR-30d-5p and BECN1 was verified by dual-luciferase reporter assay. (d) The binding sites between miR-30d-5p and XIST were predicted by Starbase (https://starbase.sysu.edu.cn/). (e) The relationship between miR-30d-5p and XIST was verified by dual-luciferase reporter assay. Quantified values were presented as mean ± standard deviation of at least three independent experiments. vs. MC. qRT-PCR: quantitative real-time polymerase chain reaction. GAPDH: glyceraldehyde-3-phosphate dehydrogenase. PAS: periodic acid Schiff reaction. DN: diabetic nephropathy. M: mimic. MC: mimic control; XIST: X inactive specific transcript.

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