LncRNA X Inactive Specific Transcript Exerts a Protective Effect on High Glucose-Induced Podocytes by Promoting the Podocyte Autophagy via miR-30d-5p/BECN-1 Axis

Figure 3

XIST targeted miR-30d-5p to regulate the viability and apoptosis of HG-induced podocytes. MPC5 cells were transfected with XIST overexpression plasmid/empty vector and or miR-30d-5p mimic/mimic control. (a-b) The expression levels of XIST and miR-30d-5p in NG or HG-treated MPC5 cells were determined by qRT-PCR. GAPDH or U6 was used as the internal control. (c–e) The expressions of XIST, miR-30d-5p, and BECN-1 in MPC5 cells transfected with XIST overexpression plasmid or empty vector were determined by qRT-PCR. GAPDH or U6 was used as the internal control. (f-g) The expressions of miR-30d-5p and BECN1 in MPC5 cells transfected with miR-30d-5p mimic/mimic control were determined by qRT-PCR. GAPDH or U6 was used as the internal control. (h–j) The first two groups of MPC5 cells were treated with NG or HG. The last four groups of MPC5 cells cotransfected with XIST overexpression plasmid/empty vector and miR-30d-5p mimic/mimic control were treated with HG. (h) The viability of MPC5 cells was determined by CCK-8 assay. (i-j) The apoptosis of MPC5 cells was determined by flow cytometry. Quantified values were presented as mean ± standard deviation of at least three independent experiments. ⁺⁺⁺ vs. vector. ^△△△ vs. MC. vs. NG.  ^, ^  ^  ^ vs. vector + MC. ^### vs. XIST + MC. ^&&, ^&&& vs. vector + M. HG: high glucose. NG: normal glucose. M: mimic. MC: mimic control. qRT-PCR: quantitative real-time polymerase chain reaction. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; XIST: X inactive specific transcript.

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