Adiponectin Gene Polymorphisms and Possible Susceptibility to Metabolic Syndrome among the Sudanese Population: A Case-Control Study

Table 1

Details of primers and restriction enzymes used in the study.

Genotypes/SNPs	Primer sequences	Annealing temperature (°C)	Amplicon size (bp)	Restriction enzymes	Digested products (bp)
(rs266729)	FP: 5ʹACT​TGC​CCT​GCC​TCT​GTC​TG-3ʹ	63.0	250	HhaI	137 + 113
11377C > G	RP: 5ʹCCT​GGA​GAA​CTG​GAA​GCT​G-3ʹ
(rs2241766)	FP: 5ʹGTGTGTTGTGGGGTCT3ʹ	56.8	305	SmaI	202 + 103
45T > G	RP: 5ʹTGT​GAT​GAA​AGA​GGC​CAG​AA-3ʹ
(rs1501299)	FP:5ʹCTACACTGATATAAACTATATGGAG3ʹ	59.4	413	BsmI	270 + 143
276G > T	RP: 5ʹCCC​CAA​ATC​ACT​TCA​GGT​TG-3ʹ

FP: forward primer; RP: reverse primer; bp: base pair.