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International Journal of Electrochemistry
Volume 2011, Article ID 340239, 8 pages
http://dx.doi.org/10.4061/2011/340239
Research Article

In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical Biosensor

1Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, 3004-535 Coimbra, Portugal
2Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey

Received 25 January 2011; Accepted 23 February 2011

Academic Editor: Bengi Uslu

Copyright © 2011 H. Eda Satana and Ana Maria Oliveira-Brett. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fludarabine, 9-β-D-arabinosyl-2-fluoroadenine, the nucleoside analog, represents a highly effective treatment for hairy cell leukemia. The electrochemical behaviour of fludarabine is an irreversible diffusion controlled oxidation mechanism and was investigated at a glassy carbon electrode in different supporting electrolytes using cyclic, differential pulse, and square wave voltammetry. The diffusion coefficient of fludarabine was calculated to be DFLU=1.71×106 cm2 s-1 in pH 7.0 0.1 M phosphate buffer. The oxidation mechanism of fludarabine occurs with the transfer of one proton and one electron and the formation of a hydroxylated species. The interaction of fludarabine with DNA was investigated, by differential pulse voltammetry, in incubated solutions and using dsDNA- and polyhomonucleotides-, poly[G] and poly[A], electrochemical biosensors. The results showed that fludarabine interacts with DNA causing changes in the DNA structure.