|
Advantages | Disadvantages |
|
(i) React only to the available fraction of metal ions; | (i) The limited understanding of the biochemistry involved. |
(ii) Are fast, less expensive, and less intensive labour; | (ii) Lack of genetic stability and short lifetime |
(iii) Are compatible with and comparable to chemical analysis; | (iii) Cells require relatively long incubation time (usually longer than 30 min); |
(iv) Are more sensitive than chemical methods; | (iv) Difficult reversibility of the signal |
(v) Produces real-time data and can be applied in field work or in situ analysis; does not involve the bulky, fragile equipment, or specialized training; | (v) Experimental conditions (temperature, pH, incubation time, buffer, and reagents) can effects the luminescence production and thus the biosensor performances |
(vi) They are more tolerant of suboptimal pH and temperatures than purified enzymes; | (vi) Less/limited of selectivity |
(vii) Are cheaper to use because the active biological component does not have be isolated and because microorganisms are living, unlimited quantities can be prepared relatively inexpensive; | |
(viii) Can provide information about the bioavailability of the analyte; | |
(xi) May perform multi-step reactions since all reactions are conveniently packaged within the cell and thus, efficiently carried out. | |
|