Interaction of Calf Thymus DNA with the Ni(II) Complex of Sodium 1,4-Dihydroxy-9,10-Anthraquinone-2-Sulphonate: A Novel Method of Analysis Using Cyclic Voltammetry

Figure 4

(a) Fluorescence spectra of (1) 200×10⁻⁶ mol dm⁻³ DNA with saturating EB (4000×10⁻⁶ mol dm⁻³) incubated for 1 hour; (2) 200×10⁻⁶ mol dm⁻³ DNA with saturating EB (4000×10⁻⁶ mol dm⁻³) incubated for 1 hour followed by addition of 3×10⁻⁶ mol dm⁻³ Na₂[Ni(NaLH)₂Cl₂] · 2H₂O; (3) 200×10⁻⁶mol dm⁻³ DNA with saturating EB incubated for 1 hour followed by addition of 3×10⁻⁶mol dm⁻³ Na₂[Ni(NaLH)₂Cl₂] · 2H₂O with incubation time 30 mins. pH = 7.4, [NaCl] = 0.500 mol dm⁻³, 25^∘C. (b) Typical double reciprocal plot for Na₂[Ni(NaLH)₂Cl₂] · 2H₂O – CT DNA interaction; [Na₂[Ni(NaLH)₂Cl₂] · 2H₂O] =23.81×10⁻⁶ mol dm⁻³, pH = 7.4, [NaCl] = 0.500 mol dm⁻³, 25^∘C.

(a)

(b)