Electrochemical Sandwich Immunoassay for the Ultrasensitive Detection of Human MUC1 Cancer Biomarker

Figure 1

Sandwich immunosensor preparation for MUC1 detection: (a) primary antibody attachment on MBs surface; (b) MBs free binding-sites blocking using BSA; (c) incubation with MUC1 antigen solutions and Ab1/MUC1 Ag complex formation on the MBs; (d) reaction with Ab2, (e) incubation with Ab3 labeled with HRP; (f) DPV measurements of MBs-bound MUC1 in presence of acetaminophen/H₂O₂ [19].